http://news.independent.co.uk/uk/science/story.jsp?dir=64&story=99903&host=3&printable=1

 

Scientists work on more effective form of vaccine

By Steve Connor Science Editor

17 October 2001

Government scientists are developing a new anthrax vaccine after encountering problems with the existing preparation, which was designed by military personnel.

Side-effects and the ineffectiveness of the current vaccine to provide long-term immunity have led scientists to experiment with a genetically engineered version.

Clinical trials of the vaccine are scheduled to begin next year, according to a spokesman for the Defence Science and Technology Laboratory (DSTL) at Porton Down in Wiltshire.

Studies at the military arm of the Porton Down research facility are understood to have identified potentially serious difficulties with the existing vaccine, which is based on a dead strain of the bacteria.

Research presented by scientists to the Fourth International Conference on Anthrax, held in Annapolis, Maryland, earlier this year, said a genetically modified vaccine could solve many difficulties, such as side-effects and the need for regular booster injections.

The vaccine is made by extracting the DNA of the anthrax microbe to isolate the gene for its protective antigen – which stimulates the human immune system – and then injecting this gene into a harmless bacterium which can be grown in fermenters to manufacture the vaccine.

A genetically engineered vaccine would be "cleaner" than the existing vaccine, which contains extraneous components of the anthrax cell that are believed to be involved in causing the side-effects seen in some inoculated people.

A Porton Down spokesman said the existing vaccine is probably able to protect against infection for a year before a booster is needed. But a clinical trial to test the ability of the British vaccine to protect humans against anthrax has never been carried out.

The spokesman said: "We've done animal studies with the anthrax vaccine and it works but it's an impossible clinical trial to do [now]. There's no objective clinical trial in individuals who otherwise would have got anthrax.

"The current vaccine works [but] it will give side-effects, and a small proportion of people will get sore arms. It needs regular boosting to remind the immune system what it looks like."

Animal studies and laboratory experiments involving tests for human antibodies suggest the current vaccine will protect most people, but the only clinical trial on humans at risk of anthrax involved an American vaccine and took place in the US in 1962.

Workers in a wool mill were involved in this trial, which compared several hundred vaccinated individuals against an equal number of unvaccinated "controls". The vaccine was found to offer effective protection against respiratory anthrax, the form of the disease that is most likely to result from a terrorist attack.

"The other problem with producing the existing vaccine is that you are fermenting anthrax bacteria," the DSTL spokesman said. The production process of a genetically modified vaccine would be safer because harmless microbes would be cultured instead.

ALL INFORMATION, DATA, AND MATERIAL CONTAINED, PRESENTED, OR PROVIDED HERE IS FOR GENERAL INFORMATION PURPOSES ONLY AND IS NOT TO BE CONSTRUED AS REFLECTING THE KNOWLEDGE OR OPINIONS OF THE PUBLISHER, AND IS NOT TO BE CONSTRUED OR INTENDED AS PROVIDING MEDICAL OR LEGAL ADVICE.  THE DECISION WHETHER OR NOT TO VACCINATE IS AN IMPORTANT AND COMPLEX ISSUE AND SHOULD BE MADE BY YOU, AND YOU ALONE, IN CONSULTATION WITH YOUR HEALTH CARE PROVIDER.