re: Influenza, Flu Vaccine and Avian Cell-Cultures

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re: Influenza, Flu Vaccine and Avian Cell-Cultures

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12508674&dopt=Abstract
 
Vopr Virusol. 2002 Nov-Dec; 47(6): 4-11. Related Articles, Links

[Cultural influenza vaccines]

[Article in Russian]

Gendon IuZ.

Recent achievements in development of influenza vaccines using long-term cell cultures MDSK or Vero are reviewed. Cell cultures were grown in bioreactors (fermenters) filled with microcarriers using serum-free culture media. Inactivated cultural influenza vaccines were clinically tested in many countries. The results of these trials showed that cultural influenza vaccines are as safe and immunogenic as conventional vaccines prepared using chicken embryos. It is emphasized that cultural influenza vaccines prepared using serum-free culture media have a number of advantages over embryonic influenza vaccines: standard preparation technology, intactness of all hemagglutinin antigen domains, absence of protein allergens and possible viral contamination inherent in chicken embryos and blood serum, and possibility of easy intensification of vaccine production in case of influenza pandemic. Taking into consideration these advantages, it may be suggested that cultural influenza vaccines will soon be widely used in medical practice.

Publication Types:


PMID: 12508674 [PubMed - indexed for MEDLINE]

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10494963&dopt=Abstract
 
Dev Biol Stand. 1999; 98: 101-10; discussion 111. Related Articles, Links

Development of a Vero cell-derived influenza whole virus vaccine.

Kistner O, Barrett PN, Mundt W, Reiter M, Schober-Bendixen S, Eder G, Dorner F.

Biomedical Research Center, Immuno AG, Orth/Donau, Austria.

Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and the presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The WHO-approved Vero cell line was used in serum-free culture to grow many influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation.

PMID: 10494963 [PubMed - indexed for MEDLINE]

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