Molecular Typing of Bordetella pertussis Isolates Recovered from Belgian Children and Their Household Members

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http://www.journals.uchicago.edu/CID/journal/issues/v36n11/30362/brief/30362.abstract.html

Clinical Infectious Diseases    2003;36:1391-1396
© 2003 by the Infectious Diseases Society of America. All rights reserved.
1058-4838/2003/3611-0006$15.00

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MAJOR ARTICLE

Molecular Typing of Bordetella pertussis Isolates Recovered from Belgian Children and Their Household Members

Iris De Schutter,1 Anne Malfroot,1 Isidoor Dab,1 Nathalie Hoebrekx,2 Gaëtan Muyldermans,2 Denis Piérard,2 and Sabine Lauwers2

1Department of Pediatrics, Pediatric Respiratory Medicine and Cystic Fibrosis Clinic, and 2Department of Microbiology, Academisch Ziekenhuis–Vrije Universiteit Brussel, Brussels, Belgium

 

Recently, a moderate increase in the prevalence of pertussis, possibly contracted from adults, has been observed among unvaccinated children. During a 3-year period, we prospectively enrolled 93 index patients with a polymerase chain reaction (PCR) and/or culture result positive for Bordetella pertussis. Among 63 household contacts of 28 index patients, PCR and culture for B. pertussis identified 25 B. pertussis–positive persons. Nineteen of 25 B. pertussis–positive household contacts were asymptomatic. Isolates were available from 10 families of both index patients and household contacts for molecular typing by pulsed-field gel electrophoresis (PFGE) and for genotyping of pertactin and pertussis toxin by sequence-specific PCR and sequencing. PFGE demonstrated homogeneity among the isolates recovered from within each family but heterogeneity among the isolates recovered from different families. B. pertussis isolates recovered from index patients and their household contacts were indistinguishable by molecular typing, demonstrating that identical strains can cause full pertussis disease in children and asymptomatic infection in adults and adolescents.

 


     Received 31 October 2002; accepted 6 February 2003; electronically published 21 May 2003.
     Financial support: Onderzoeksraad, Vrije Universiteit Brussel, Brussels, Belgium (grant 624).

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