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Infection and Immunity, June 2003, p. 3402-3408, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3402-3408.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
 

Disparity in Functional Activity between Serum Anticapsular Antibodies Induced in Adults by Immunization with an Investigational Group A and C Neisseria meningitidis-Diphtheria Toxoid Conjugate Vaccine and by a Polysaccharide Vaccine

Shannon L. Harris,¹ Adam Finn,² and Dan M. Granoff^1*

Children's Hospital, Oakland Research Institute, Oakland, California 94069,¹ Institute of Child Health, Bristol, United Kingdom²

Received 23 December 2002/ Returned for modification 19 February 2003/ Accepted 11 March 2003

Polysaccharide-protein conjugate vaccines elicit higher concentrations of serum anticapsular antibody in infants and children than do unconjugated polysaccharide vaccines. The conjugate-induced antibodies also have higher avidity and complement-mediated bactericidal activity. Similar vaccine-related differences in the magnitude or functional activity of antibody are observed infrequently in immunized adults. We compared the antibody responses of adults immunized with an investigational group A and C meningococcal conjugate vaccine to those elicited by an unconjugated meningococcal polysaccharide vaccine. Although there were no significant differences between the respective geometric mean bactericidal titers of the two vaccine groups, it took, on average, three- to fourfold higher concentrations of polysaccharide-induced serum anticapsular antibody to achieve 50% complement-mediated bacteriolysis than conjugate-induced antibody (P < 0.001 for groups A and C). At limiting doses, the polysaccharide-induced anticapsular antibodies also were less effective in conferring passive protection against meningococcal bacteremia in infant rats challenged with a group C strain (P < 0.04). The avidity index of the group C antibodies was higher in the conjugate vaccine group than in the polysaccharide vaccine group (P < 0.005). The disparities in the functional activity of the anticapsular antibodies elicited in adults by the two vaccines imply fundamental differences in the respective B-cell populations stimulated.

* Corresponding author. Mailing address: 5700 Martin Luther King, Jr. Way, Oakland, CA 94609. Phone: (510) 450-7640. Fax: (510) 450-7915. E-mail: dgranoff@chori.org.

Editor: D. L. Burns

Infection and Immunity, June 2003, p. 3402-3408, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3402-3408.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

 

Copyright © 2003 by the American Society for Microbiology. All rights reserved.