Expression of Immunogenic S1 Glycoprotein of Infectious Bronchitis Virus in Transgenic Potatoes

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http://jvi.asm.org/cgi/content/abstract/77/16/9090

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Expression of Immunogenic S1 Glycoprotein of Infectious Bronchitis Virus in Transgenic Potatoes

Ji-Yong Zhou,¹ Jian-Xiang Wu,¹ Li-Qin Cheng,¹ Xiao-Juan Zheng,¹ Hui Gong,¹ Shao-Bin Shang,¹ and En-Min Zhou^2*

Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China,¹ Department of Veterinary Diagnostic and Productive Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011²

Received 19 February 2003/ Accepted 19 May 2003

The expression of infectious bronchitis virus (IBV) S1 glycoproteinin potatoes and its immunogenicity in mice and chickens were investigated. Potato plants were genetically transformed witha cDNA construct encoding the IBV S1 glycoprotein with the Agrobacteriumsystem. Genomic DNA and mRNA analyses of the transformed plantletsconfirmed the integration of the foreign cDNA into the potato genome, as well as its transcription. Mice and chickens vaccinated with the expressed IBV S1 glycoprotein produced antibodies that neutralized IBV infectivity. After three immunizations, vaccinated chickens were completely protected from virulent IBV infection. These results demonstrate that transgenic potatoes expressingIBV S1 glycoprotein can be used as a source of recombinant antigenfor vaccine production.

* Corresponding author. Mailing address: Department of Veterinary Diagnostic and Production Animal Medicine, 2630 Vet. Med. Building, College of Veterinary Medicine, Iowa State University, Ames, IA 50011. Phone: (515) 294-4699. Fax: (515) 294-3654. E-mail: ezhou@iastate.edu.

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